The Smithsonian Institution’s museums and research units have been working to address the challenges posed by contaminated museum collections and, to that end, organized the Pesticide Mitigation in Museum Collections Workshop to learn more about potential approaches for remediation of contaminants. The papers assembled here result from the workshop and represent experimental attempts to use a wide assortment of mitigation and remediation technologies on simulated and real cultural objects. These and other theoretical approaches hold great promise for decontamination of collections for the safety of all who work with them.