Fourier transform Raman spectra of human keratotic biopolymers were obtained. The spectra from skin stratum corneum, callus, nail and hair were fluorescence free and the samples did not degrade during analysis. Assignments consistent with the vibrational wavenumbers were made to characterize the molecular and conformational nature of the samples. The principal structure dissimilarities were in the sulphur content of the tissues containing hard keratin (hair and nail) and soft keratin (stratum corneum and callus).