This study assesses different organic separators used as protection in the process of moulding originals, in this case, for use in reproduction of the female terracotta bust in Puig des Molins, Ibiza (Punic age, 3rd Century B.C.). The use of an inappropriate separator could cause chromatic changes and the presence of residues on the surface which are difficult to remove. Different separators have been tested to this end (polyvinyl alchol Gelvatol by Agar Agar, latex Rubber latex 602 by CTS, emulsifying oil Silical 100 destacante by CTS and the Cyclododecane CDD by CTS) applied on test pieces with characteristics similar to the original. After elimination of the separators and the elastomer, the test pieces were furthermore subjected to accelerated ageing test by UV light irradiation for 360 hours, with the sole purpose of observing the possible chromatic changes and/or surface modifications that these had undergone. The different test pieces were observed under the optical microscope (OM) and the visible spectrophotometer (colorimeter) and in the infrared spectroscope by Fourier transform (FT-IR). The results obtained have shown that the dual latex/polyvinyl alcohol layer has excellent workability properties, accuracy in the impression, dual barrier/protection effect and reversibility, without any presence of residues. Hence for the present case studied, the use of the dual layer has come forward as the most ideal separator during the moulding process.