The stable isotopic analysis of archaeological and paleontological bones has become a common method to examine questions of ecology, climate, and physiology. As researchers addressing such questions incorporate museum collections in their studies, it is necessary to understand the isotopic effects of common preservation techniques utilized in such collections to ensure the preservation of original isotopic values. This study examines the effects of PVAc glue (polyvinyl acetate) applied in acetone solution and the subsequent removal of PVAc using various organic solvents on the [delta]13C and [delta]15N values of extracted bone collagen, the [delta]13C and [delta]18O values of carbonate in bone hydroxyapatite, and the [delta]18O values of phosphate in hydroxyapatite. The data demonstrate that isotopic values in the collagen and phosphate are unaffected by any combination of PVAc treatment and solvent application. The carbonates show little variation in [delta]13C values, but exhibit variable [delta]18O values upon exposure to the PVAc solution. It is here suggested that [delta18]O values from carbonates in PVAc-treated bones do not retain an original isotopic value and should not be included in future studies.