Two characters (antennal configuration and the last abdominal segment) were found to be reliable guides in sexing pupae of Trogoderma spp. Pupae were exposed by gently pressing the center of the ventral side with a small brush; then, with the large insect pin, the head region of the last larval skin was lifted and pulled back, thus exposing the exarate-type pupae. As in adults, the exposed antennal structures are distinctly different among species, and between male and female pupae of each species. The last abdominal segment serves as an accurate and rapid key for sexing pupae, because in the male pupae the last segment is round, while females have a distinct pair of processes on the last abdominal segment. The handling of pupae had no deleterious effects on the reproductive capabilities of the adults.