Fourteen Taiping Heavenly Kingdom murals in Tangzijie in Nanjing had been preservation before. In order to make the murals in a better condition for exhibition, on-site pre-protection is done, the murals are moved, conserved and restored in laboratory based on comprehensive on-site survey and restoration information collection. Paraloid B72 and Primal AC33 as mature protective materials for the murals were used to reinforce the murals. Steam cleaning and laser cleaning were introduced to the mural’s surface cleaning. Pasting wood pulp paper and pre-brushing ethanol were also employed as methods to improve the repair technique. A good result is achieved after conservation and restoration of the murals. The process can provide references for similar murals.