Bitumen used as a preservative in ancient Egyptian mummies was previously thought to come only from the Dead Sea in Palestine. Other, closer sources of bitumen were investigated at Abu Durba and Gebel Zeit on the shores of Egypt’s Gulf of Suez. Bitumen from these localities and from five mummies was analyzed using molecular biomarks derived from gas chromatography/mass spectrometry. It was found that four of the mummies contained Dead Sea bitumen, and the fifth and oldest (900 BC) had bitumen from Gebel Zeit, thus providing the first evidence for the use of an indigenous source of bitumen in ancient Egypt.