For the study of art objects and their restoration, the identification of binding media as well as that of pigments is considerably important. Binding media consist of complicated organic compounds but only a small amount of sample can be used for analysis. For this reason analysis of binding media by means of conventional techniques has been difficult. In this paper, identification of protein containing binders such as glue used in Buddhist wooden statues was carried out by sodium dodecylsulfate polyacrylamide gel electorophoresei(SDS-PAGE) and by high performance liquid chromatography (HPLC). By using SDS-PAGE, it was not possible to distinguish mammal glue from fish glue whereas by HPLC these two types of glue were clearly distinguished.