In a course of the study on the preparation of an Egg Tempera, this report presents the results of the absorption and desorption on the preparation. Preparation were prepared by painting the calcium salts had mixed the melting glue in warm water, on the support. When this preparation were dried up, there were 1 mm in thickness and about 2 grams one test piece. At 100 percent RH, 86. 4 percent RH and 64.4 percent RH, the preparation in each humidity showed a good deal of absorption the first three days. And the preparation had absorbed in full were much of desorbed, first three days at 86.4 percent RH and 64.4 percent RH. The pigments had kneaded the vehicles and painting on the preparations showed the different absorptions. If we are careless the Egg Tempera is attacked by the microorganisms for the preparation absorbed.