The study of the air-spora in the Central Library of Gorakhpur University indicated the presence of 19 different types of fungal spores. Amongst these <i>Aspergillus niger, A. flavus, Cladosporium</i> spp., <i>Curvularia</i> spp., <i>Paecilomyces</i> sp., <i>Penicillium</i> spp., and <i>periconia</i> sp. were more than 80. 5% followed by species of * 2Absidia, Alternaria, Chaetomium, Mucor, Rhizopus, Trichophyton*1, and <i>Cercospora</i>. Fungal spores showed time trends during the study period. The fungal spore concentrations gradually increased from July to September, and attained their maximum in September to October, and thereafter decreased. Besides fungal spores, small pieces of paper, dust particles, and insect derivatives loaded with fungal mycelium and bacterium were also observed throughout the investigation period. -- AATA