23574
Accession Number
654
Author
Kirchner, J.G.; Miller, John M.; Keller, G.J.
Title Of Article Chaper
Separation and identification of some terpenes by a new chromatographic technique
Title Of Journal Book
Analytical chemistry
Volume
23
Issue
3
Pages
420-425
Reference Bibliography
Includes bibliog. refs.
Language Of Text
English
Literature Type
Serial
Literature Level
Analytic
Abstract
A chromatographic method for separating terpenes was developed for the determination of the volatile flavoring constituents of citrus fruit. This new technique in organic chromatography has been introduced by using adsorbent-coated glass strips in a manner analogous to paper chromatography. After the mixture has been spotted near one end of the strip, the chromatogram was developed with the aid of capillary attraction by dipping in a suitable solvent. The solvent was then evaporated from the strip and the various zones were indicated by spraying with suitable reagents. On spraying with a fluorescein solution and exposing to bromine vapor, compounds which absorb bromine faster than the fluorescein show up as yellow spots on a pink background. Very unreactive compounds can be located by spraying with a concentrated sulfuric-nitric acid mixture and heating to cause charring of the compounds. The technique can be applied to other types of compounds and is a rapid method of checking solvents and adsorbents for use on larger chromatographic columns. The term chromatostrip has been suggested for application to these absorbent-coated glass strips. -- CCI
pub_id
23574