By analysis of the quake harm for Shanghai and its neighborhood area, combining the results in Shanghai area earthquake researth, according to the real requirements of the cultural relics protection from quake for Shanghai Museum, the fortify level and the motion parameters of the cultural relics protection from quake from museum have been studied and determined. The input acceleration peak level of the site motion input peak acceleration level be determined as the exceed probability 3% by 50 years, the motion response spectra be adopted by the quake influence coefficient curve (damping ratio 0.05) from the Shanghai architecture anti-seismic code (DGJ08-9-92), and the motion input peak acceleration be adjusted to 180 gal. In this way, one side the enough safety be provided and another side the quake background of Shanghai Museum building site be considered. Then here adopt the global method by fitting response spectra, ie. by fitting motion response standard spectra selected, synthesized 6 artificial wave time history curves. The fitting precision is to be controlled in 5% about the relative error. These artificial quake wave histories have the same statistical characteristics, along with one real motion time history in the Shanghai architecture anti-seismic code (DGJ08-9-92), as the input motion of the structural quake response analysis for Shanghai Museum building. Then according to the analysis of the structural characteristics for Shanghai Museum, combining with the real measurement to the building structure dynamics characteristics, the structure design parameters have been corrected. By the story modal structural time history analysis and computation method, computed and gained the quake motion response at the every floor in the Shanghai Museum building when it encounters the fortify quake. The computational results show that under the action of the fortify quake, the quake motion response is provided with obvious story amplify effect in the every story of the Shanghai Museum building. For the quake motion response by the computation to the every story, work out the statistical analysis and give out the motion parameters for the every story in the Shanghai Museum building. It can be as the basis for the cultural relics quake harm analysis, isolator system design and other protection methods future.