Reusable, flexible enclosures and high-volume nitrogen supplies for a large scale insect eradication treatment were investigated. The treatment system consisted of a high-volume nitrogen source, a gas humidification module, an anoxia enclosure or tent, environmental sensors, and a vacuum pump. A 10 cube meter volume reusable tent was fabricated from Filmpak 1193 film with an oxygen leak rate of less than 0.005 percent (50 ppm) per day. Thus, it could maintain the anoxic condition with no nitrogen flow for several weeks once purged to a 0.1 percent oxygen level. A commercially available 6 cube meter bubble enclosure from Rentokil had a leak rate low enough to be maintained at 0. 3 percent oxygen by a moderate constant flow of nitrogen. Liquid nitrogen and a nitrogen generator were successfully tested as nitrogen sources for large scale anoxia systems. A humidification module was successfully redesigned for improved accuracy and controllability of relative humidity at both low and high nitrogen flow rates. Safety issues related to large scale applications of nitrogen are discussed. -- ICCROM