Fabrics were designed and constructed from hydrophilic and hydrophobic spun yarns to give planar substrates containing amphiphilic microchannels with defined orientations and locations. Fluid transport was measured and compared in fabric constructions with systematic variations in the numbers of adjacent parallel and orthogonal hydrophilic yarns. The successful control of finite liquid volumes was demonstrated, indicating that careful design of amphiphilic fabrics can be used to create simple microfluidic structures for various end-use applications. Simultaneous co-flow of an aqueous and hydrocarbon fluid was also demonstrated. This result, combined with the high degree of interfacial contact and micron-scale diffusion lengths of such co-flowing immiscible fluid streams inside amphiphilic fabrics suggest potential applications as highly scalable and affordable microcontactors for industrial-scale liquid-liquid extractions.