The efficiency of rinsing two different alpha-amylases out of paper after conservation treatment was measured using radioactively labeled enzymes. The radioactive enzymes were used to treat small samples of Whatman and Japanese papers. The papers were subjected to various rinsing procedures, and the radioactivity remaining in the paper samples was determined. Buffers were used to maintain the pH of the enzyme solutions, and their effect in the removability of the enzymes was analyzed. Two water rinses were shown to be sufficient in removing more than 99per thousand of both enzymes tested under optimal conditions. It was also found that increasing the concentration of the enzyme solution resulted in an increase in retention of enzymes in the paper