9122
Title Of Article Chaper
Ultraviolet-Fluorescence Microscopy of Paint Cross Sections: Cycloheptaamylose-Dansyl Chloride Complex as a Protein-Selective Stain
Title Of Journal Book
Journal of the American Institute for Conservation
Volume
31
Issue
3
Pages
267-274
Collation
8 p. : ill.
Reference Bibliography
Includes bibliographical references
Publisher
American Institute for Conservation of Historic and Artistic Works
Publisher City
Washington
ISSN
0197-1360
Language Of Text
English
Literature Type
Serial
Literature Level
Analytic
Abstract
Cycloheptaamylose-dansyl chloride complex, a new fluorochrome- specific for proteins, has been investigated as a stain to identify artists' paint media. Its selectivity and limitations are compared to those of the following commonly available fluorescent and nonfluorescent stains: lissamine rhodamine B sulfonyl chloride, 5- fluorescein isothiocyanate, amido black, Sudan black, rhodamine B, and 2',7'-dichlorofluorescein. The methods used to examine the characteristics of these stains were reflected visible light microscopy and ultraviolet-fluorescence microscopy. The known samples of artists' media analyzed with the stains were linseed oil, casein, tempera, glair, gelatin, rabbit skin glue, and acrylic emulsions. -- CAL
Keywords
UV;ultraviolet;fluorescence;microscopy;paint;protein;stain -- CAL
pub_id
9122