8493
Accession Number
17004
Title Of Article Chaper
Effect of fluorescent-labelled lectins on visualization of decay fungi in wood sections
Title Of Journal Book
Phytopathology
Volume
75
Issue
3
Pages
329-332
Collation
11 refs., 2 figs., 2 tables
Language Of Text
English
Literature Type
Serial
Literature Level
Analytic
Abstract
Fluorescent-labelled lectins were used to stain white, brown and soft rot fungi in microtomed sections of wood from several coniferous species; the sections were then observed with incident fluorescence microscopy. Fluorescein-labelled wheat germ agglutinin greatly enchanced hyphal visibility of all fungi over that obtained with the conventional safranin-O/picro-aniline blue method. Thus, fluorescent-labelled lectins provide an improved tool for observing fungi in wood sections at stages when detection with conventional light microscopy varies, and they could prove useful for following the course of decay from spore germination to complete wood breakdown. -- AATA
pub_id
8493