Thousands of Greek and Latin stamped handles have been found at various excavations in Israel. These handles belong to large amphorae in which a variety of foodstuffs were shipped from Greece, Anatolia, and Italy. When the amphorae have stamps which can be traced to specific dates in the country where they originated, they are valuable for dating stratigraphical levels in each excavation in which it is found. The purpose of this study of Hellenistic and Roman stamped jars was to establish the existence of dated trade relations between ancient Jerusalem and distant countries. Instrumental neutron activation analysis of stamped handles excavated in the City of David and comparison with reference material from Rhodes and Thásos indicated that most of the stamps originated in Rhodes, one in Kos, and another (the Zenon stamp) was probably manufactured in Knidos or Kos. Since both are only 15 kilometers apart, more sampling from these geographical regions is required.