Many American museums installed period rooms in the early twentieth century. Eighty years later, different environmental standards and museum expansions mean that some of those rooms need to be removed and either re-installed or placed in storage. Over the past four years the Museum of Fine Arts, Boston has de-installed all of their European and American period rooms as part of a Master Plan to expand and re-organize the museum. The removal of the rooms was coordinated and supported by museum staff and performed by private contractors. The first part of this paper will discuss the background of the project and the particular issues of the museum to prepare for the deinstallation. The second part will provide an overview of the deinstallation of one specific, painted and fully-paneled room to illustrate the process. It will include comments on the planning, logistics, physical removal and documentation, as well as notes on its future reinstallation.