In order to protect the original color layer of the Portrait of Emperor Rudolph II by G. Arcimboldi (1527-1593) the removal of overpaint with organic solvents was not possible. Therefore a new multienzyme preparation isolated from Antarctic krill (<i>E. superba* 1) was used. Unlike the common industrial enzymes, this natural mixture, containing both endo and exopeptidases, ensures an extensive break-down of complex proteinous substrates to free, water soluble amino acids. The application of krill enzymes gave excellent results: the overpainting as well as varnish residues were rapidly removed, while the original oil-based colors remained intact. -- AATA